Journal: The Journal of Physiological Sciences : JPS
Article Title: Changes in adrenoceptor expression level contribute to the cellular plasticity of glioblastoma cells
doi: 10.1016/j.jphyss.2025.100016
Figure Lengend Snippet: Glioblastomas alter their adrenoceptor expression levels depending on their stage of differentiation. (A). Number of RNA sequence reads representing the expression of mRNAs encoding adrenoceptors in GSCs and DGCs of MGG4, MGG6, and MGG8. ADRA1D , ADRA2C , ADRB1 , and ADRB2 , which are well expressed and particularly variable, are highlighted with orange lines. (B). Graph showing the relative expression levels of ADRA1D , ADRA2C , ADRB1 , and ADRB2 in GSCs and DGCs of MGG4, MGG6, and MGG8 as a ratio of DGCs to GSCs. The vertical axis is shown in logarithm; of the four genes, those representing a common pattern of variation in the three cell lines are highlighted with green lines. (C). Western blotting results evaluating protein expression of ADRA1D, ADRB1, and ADRB2 in GSCs and DGCs of MGG4, MGG8, MGG18, and MGG23. (D). Immunostaining images showing changes in ADRA1D expression in GSCs and DGCs of MGG8. (E). Immunostaining image showing ADRA1D expression in tumor tissue of orthotopic xenografts composed of 8GSC-RFP and 8DGC-GFP. ADRA1D, which is upregulated in the differentiated state, is positive in some RFP-positive cells (formerly GSC cells) and negative in some GFP-positive cells (formerly DGC cells), indicating that, like undifferentiated and differentiated markers, the expression pattern of ADRA1D is also heterogeneous. Staining for ADRA1D in normal mouse brain tissue is shown as a control.
Article Snippet: ADRB2 Rabbit Poly Ab , 1:2000 , Proteintech , 13096 −1-AP.
Techniques: Expressing, Sequencing, Western Blot, Immunostaining, Staining, Control
Proteintech is a verified supplier 
